Anti-GAMT Antibody

GAMT (guanidinoacetate N-methyltransferase) is the enzyme that completes creatine biosynthesis by methylating guanidinoacetate using S-adenosylmethionine, and altered expression is implicated in cerebral creatine deficiency syndromes. This unconjugated polyclonal reagent from Antibodies.com was raised in rabbit and is supplied as a 100 µl vial at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol. It is validated for western blot, immunohistochemistry, and immunofluorescence in human, mouse, and rat samples, supporting studies of hepatic methyl-group metabolism, tissue-specific expression profiling, and GAMT knockout or knockdown phenotypes. For best performance, aliquot the antibody upon arrival and store at -20 °C, avoiding repeated freeze-thaw cycles; briefly centrifuge the tube before opening to recover the full volume. Researchers working on mitochondrial one-carbon metabolism, in vitro hepatocyte models, or in vivo rodent models of creatine depletion can pair this reagent with appropriate HRP- or fluorophore-conjugated secondary antibodies for chromogenic or fluorescent detection on fixed sections and immunoblots.