Anti-GALK1 Antibody

Raised in rabbit and affinity-purified against galactokinase 1, this unconjugated polyclonal reagent from Antibodies.com targets the cytosolic GALK1 enzyme that catalyzes the first committed step of the Leloir pathway, phosphorylating galactose to galactose-1-phosphate. The antibody is validated across multiple research workflows including Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and ELISA, with confirmed reactivity against human, mouse, and rat samples for comparative expression studies across model organisms.

Supplied as 100 µl of polyclonal antiserum in a single-use vial, the reagent pairs readily with labeled secondary detection systems of the researcher's choice, supporting flexible assay design in fluorescence, chromogenic, or chemiluminescent readouts. Because GALK1 sits at a metabolic branch point relevant to galactosemia research, hepatic carbohydrate handling, and broader galactose metabolism investigations, this antibody is useful for expression profiling, tissue distribution mapping, and pathway-focused studies. Refer to the lot-specific data sheet included with the shipment for recommended starting dilutions, antigen retrieval conditions appropriate for IHC, and blocking guidance for clean immunofluorescence staining.