Anti-GABRA1 Antibody

This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against the alpha-1 subunit of the human GABA-A receptor (GABRA1), a ligand-gated chloride channel that mediates fast inhibitory synaptic transmission throughout the central nervous system. The antibody is validated for Western blot (WB) and immunohistochemistry (IHC) workflows, allowing researchers to examine endogenous receptor expression in tissue lysates and fixed brain sections.

GABRA1 is the most abundantly expressed alpha subunit of the GABA-A receptor family and is frequently studied in the context of epilepsy, anxiety, sedation pharmacology, and neurodevelopmental disorders. The polyclonal format increases the chance of recognising the target across multiple epitopes, which is useful when probing subtle changes in subunit expression or subcellular localisation in neurones and glia. The 100 µl volume supports multiple experimental runs, and aliquoting on arrival helps protect activity across freeze-thaw cycles.

For Western blot, resolving 20-40 µg of total protein from neural tissue or transfected cell lines gives a clear receptor band near the expected molecular weight. For IHC, the antibody can be applied to formalin-fixed paraffin-embedded or frozen sections using standard citrate or EDTA antigen retrieval, followed by HRP or fluorescence-based detection. Store at -20°C in single-use aliquots to preserve reagent performance over time.