Anti-FYN (phospho Y416) + Lck (phospho Y416) Antibody

The dual-specificity polyclonal antibody from Antibodies.com recognizes the conserved phospho-tyrosine 416 activation-loop epitope shared by the Src-family kinases FYN and Lck, enabling simultaneous detection of their activated states in cell-signaling studies. Raised in rabbit and supplied as an unconjugated IgG preparation, the reagent is provided in 200 µl aliquots calibrated for Western blot workflows probing phospho-Src-family activity downstream of T-cell receptor engagement and related tyrosine-kinase-driven pathways. Reactivity has been validated against human and mouse samples, supporting investigations into lymphocyte activation, thymic development, and neuronal signaling in which these two kinases play non-redundant roles.

Each vial is intended for research use only and should be stored refrigerated to preserve phospho-epitope binding activity across multiple freeze-thaw cycles; aliquoting upon first thaw is recommended to limit repeated temperature cycling. The antibody targets the autophosphorylation site corresponding to Y416 in the kinase activation loop, a marker widely adopted to report catalytically active FYN and active Lck in lysates prepared from immune and neural cell lineages.