Anti-FucT-III Antibody

This rabbit polyclonal antibody targets fucosyltransferase III (FUT3/FucT-III), a Golgi-resident enzyme that transfers fucose from GDP-fucose onto type 1 and type 2 precursor chains during Lewis blood group antigen biosynthesis. Supplied at 1 mg/ml in PBS with 0.05% sodium azide (approx. pH 7.2), the unconjugated reagent from Antibodies.com is validated for Western blot, immunohistochemistry, and immunocytochemistry workflows. The 100 µl pack size supports repeated staining runs; to preserve activity, store refrigerated at 4 °C and aliquot on first use to avoid repeated freeze-thaw cycles of the stock. Published reactivity covers human, mouse, and rat samples, enabling comparative glycobiology studies across these three species. Because FUT3 drives cell-surface α(1,3/1,4)-fucosylation that is frequently altered in epithelial carcinomas, this reagent is useful for investigating tumor-associated glycosylation changes, blood group antigen expression on fixed tissue sections, and fucosylation patterns in cultured cell monolayers. The polyclonal format provides multi-epitope recognition, helping detection of low-abundance FUT3 in routine immunoblotting and ICC preparations.