Anti-FRK Antibody

This polyclonal antibody from Antibodies.com is raised against FRK (Fyn-Related Kinase), a non-receptor tyrosine kinase of the Src family that participates in intracellular signaling cascades regulating cell proliferation, adhesion, and differentiation. Supplied as an unconjugated reagent in a 100 µl vial, the reagent is ready for direct labeling or for use in standard reporter-based detection workflows across multiple techniques.

Validation has been performed for western blot, immunohistochemistry, and immunofluorescence, giving researchers flexibility when localizing FRK expression in tissue sections, cultured cells, or fractionated lysates. Reactivity has been confirmed in human, mouse, and rat samples, supporting comparative studies across common model organisms. The polyclonal format recognizes multiple epitopes on FRK, which can improve sensitivity when target abundance is low or when working with denatured proteins.

For western blot, lysates prepared in standard RIPA or SDS-containing buffers can be separated by SDS-PAGE and probed after transfer to nitrocellulose or PVDF membranes. Immunohistochemistry on formalin-fixed paraffin-embedded sections typically benefits from antigen retrieval in citrate or EDTA buffer. The unconjugated format allows laboratories to choose their preferred reporter chemistry, whether HRP, biotin, or fluorescent conjugates. Shipped as a liquid formulation, the antibody should be stored refrigerated at 4°C for short-term use, with aliquoting recommended to limit freeze-thaw cycles in glycerol-containing buffer. For research use only.