Anti-Frizzled-5 (Y500) Antibody

Available from Antibodies.com as a primary antibody reagent, this unconjugated rabbit polyclonal antibody targets Frizzled-5 and is formulated at 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. The reagent is validated for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF/ICC) applications, with reactivity confirmed against Human, Mouse, and Rat samples.

The antibody was raised against a synthetic peptide corresponding to amino acids 468-519 of Human Frizzled-5 and specifically detects endogenous levels of the Frizzled-5 protein, which has a reported molecular weight of ~ 65 kDa. The reagent is affinity-purified from rabbit antiserum by affinity-chromatography using an epitope-specific immunogen, achieving a purity of > 95% as determined by SDS-PAGE.