Anti-Frizzled-4 Antibody

Offered by Antibodies.com, this rabbit polyclonal primary antibody recognizes Frizzled-4 (FZD4), a member of the Frizzled family of Wnt receptors involved in Wnt/β-catenin signaling. The reagent is supplied as a 50 µL liquid aliquot formulated at approximately 1 mg/mL in phosphate buffered saline (PBS), pH 7.3, with 0.05% sodium azide as a preservative, and is unconjugated for maximum flexibility in downstream detection. It is raised in Rabbit host, is of polyclonal clonality (IgG isotype), and was generated against a recombinant fusion protein corresponding to amino acids 37–222 of human FZD4 (NP_036325.2). The antibody is validated for Western blot (WB) applications with a recommended dilution range of 1:500–1:2,000 and shows broad reactivity with Human, Mouse, and Rat samples, making it suitable for cross-species studies of Frizzled-4 expression in cell and tissue lysates. For optimal preservation of performance, the product should be stored short-term at 4 °C upon receipt and aliquoted for long-term storage at –20 °C, with repeated freeze/thaw cycles avoided. Researchers studying Wnt pathway biology, angiogenesis, retinal vascular development, or FEVR-related signaling will find this affinity-purified reagent a reliable tool for detecting endogenous Frizzled-4 protein levels.