Anti-Frizzled-4 Antibody

Raised in rabbits against the human Frizzled-4 (FZD4) receptor, this polyclonal unconjugated primary antibody from Antibodies.com is supplied at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide, at an approximate pH of 7.3. The antibody is validated for western blot detection of Frizzled-4 and has been confirmed to react with human, mouse, and rat samples, supporting comparative studies across commonly used mammalian models.

Frizzled-4 is a seven-transmembrane Wnt co-receptor best known for its role in the Norrin/β-catenin signaling axis controlling retinal angiogenesis and blood-retina barrier integrity. Researchers studying FEVR (Familial Exudative Vitreoretinopathy), Wnt-driven angiogenesis, planar cell polarity signaling, and vascular development in the eye and inner ear commonly use anti-FZD4 antibodies to characterize receptor expression in transfected cell lines and primary tissue lysates.

Each vial contains 100 µl of ready-to-dilute antibody. For short-term use, store at 4°C; for long-term stability, aliquot and freeze at -20°C, avoiding repeated freeze-thaw cycles. The polyclonal format provides recognition of multiple epitopes on Frizzled-4, which can improve detection in samples where post-translational modification or splice variation might mask a single epitope site.