Anti-FOXP3 Antibody

Immunology laboratories rely on this rabbit polyclonal primary antibody from Antibodies.com to detect FOXP3, the forkhead-box transcription factor that defines CD4+CD25+ regulatory T cells (Tregs). FOXP3 sits at the Xq11.23 locus on the X chromosome, and loss-of-function mutations cause IPEX syndrome, making reliable detection of this nuclear protein a routine requirement in transplant tolerance, autoimmunity, and tumor immunology research.

The reagent is supplied as 100 µL of unconjugated rabbit IgG, affinity-purified for broader epitope coverage than a single monoclonal clone. It has been validated for western blot, immunohistochemistry, and immunoprecipitation on human, mouse, and rat samples, supporting matched work with patient-derived material and rodent disease models. Common pairings include CD4 and CD25 surface markers when sorting Tregs from lymphoid tissue, and Foxp3-reporter lines in mouse colitis or tumor models where Treg infiltration correlates with disease outcome.

Because FOXP3 is nuclear and regulated both transcriptionally and post-translationally, the antibody performs best on freshly isolated or carefully fixed material; aliquot on first use and store refrigerated to preserve activity across repeat experiments. The unconjugated format leaves the choice of HRP, biotin, or fluorophore conjugation entirely to the end user, making this Antibodies.com reagent flexible across standard detection workflows from immunoprecipitation to imaging.