Anti-FoxO4 (phospho-S197) Antibody

Phosphorylation of the FoxO4 transcription factor at serine 197 is a regulatory post-translational modification linked to AKT/SGK signaling cascades, oxidative stress response, and nucleocytoplasmic shuttling in mammalian cells. This rabbit polyclonal antibody from Antibodies.com has been raised against the phospho-S197 epitope and selectively recognizes the phosphorylated form of FoxO4, allowing researchers to distinguish activation-state dynamics from total FoxO4 protein levels in experimental samples.

The reagent is supplied as an unconjugated whole antiserum at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2. Each vial contains 100 µL of the polyclonal antibody stock. Sodium azide is included as a preservative; users performing live-cell imaging should note that azide-containing buffers are not compatible with those workflows.

Validated applications include western blot for detection of phospho-FoxO4 in cell and tissue lysates, and immunohistochemistry on formalin-fixed paraffin-embedded sections. The antibody cross-reacts with FoxO4 from human, mouse, and rat origin, supporting translational studies that span cell line models and rodent tissue specimens. Because the reagent is phospho-specific, recommended sample handling includes fresh or snap-frozen lysates prepared with phosphatase inhibitors to preserve the S197 modification; aliquot the antibody upon first thaw and avoid repeated freeze-thaw cycles to maintain reagent activity.

Recommended working dilutions should be determined empirically by the user for each assay, with detection typically achieved using a horseradish peroxidase- or fluorophore-conjugated secondary antibody appropriate for the host species (rabbit). The clonality is polyclonal and the antibody is unconjugated, enabling flexible secondary reagent selection or downstream labeling strategies.