Anti-FOXO3 (phospho S253) Antibody

Phosphorylation of the forkhead box O3 transcription factor at serine 253 is a key regulatory event controlled by the PI3K-AKT signaling cascade, and this rabbit polyclonal antibody from Antibodies.com enables direct detection of that post-translational modification. Raised against a synthetic phosphopeptide corresponding to the S253 site, the reagent is supplied as 50 µl of unconjugated IgG in a vial format and is validated for western blot, immunohistochemistry, and immunofluorescence workflows across human, mouse, and rat samples.

Researchers studying insulin signaling, oxidative stress responses, autophagy regulation, and apoptosis pathways use phospho-S253-specific detection to monitor AKT-dependent cytoplasmic sequestration of FOXO3. The polyclonal format provides broader epitope coverage for capturing the modified residue in denatured and fixed sample preparations. Storage at -20 °C in small aliquots helps preserve phospho-specific binding activity over multiple freeze-thaw cycles, and the unconjugated format gives laboratories flexibility to pair the antibody with HRP, biotin, or fluorophore conjugates of their own choice.