Anti-FOXO3 Antibody

This polyclonal antibody from Antibodies.com targets human FOXO3, a Forkhead box transcription factor that regulates genes controlling apoptosis, autophagy, oxidative-stress responses, and glucose metabolism. Raised in rabbit and purified as an IgG preparation, the reagent is supplied unconjugated in a 200 µl vial, giving laboratories flexibility for direct conjugation or for use with standard HRP, biotin, or fluorescence-labeled secondary reagents.

Validated applications are Western blot (WB) and immunohistochemistry (IHC) on human samples. In Western blot, the antibody detects the FOXO3 protein band and can resolve phosphorylation-driven mobility shifts associated with AKT and SGK kinase signaling. In IHC performed on formalin-fixed, paraffin-embedded tissue sections, it highlights nuclear and cytoplasmic FOXO3 distribution, supporting studies of tumor-suppressor activity, cellular stress responses, and longevity-related pathways.

Researchers working on insulin/IGF-1 signaling, caloric restriction mimetics such as rapamycin, age-related disease, or mechanisms of chemoresistance rely on FOXO3 readouts to connect upstream kinase activity with downstream transcriptional programs. The unconjugated IgG format suits chromogenic, fluorescent, and chemiluminescent workflows depending on the chosen detection antibody, making it a versatile tool for cell biology, cancer research, and immunology laboratories.