Anti-FOXO1 (phospho S256) Antibody

Supplied by Antibodies.com as a 50 µl vial, this rabbit polyclonal IgG reagent selectively recognizes FOXO1 only when phosphorylated at serine 256. The phospho-S256 epitope sits within the AKT/PKB consensus motif, the regulatory switch that drives FOXO1 nuclear export and shutdown of its pro-apoptotic transcriptional program downstream of insulin, IGF-1, and other growth factor inputs. Researchers deploy it in Western blot workflows to track phosphorylation dynamics in lysates from cell culture and tissue samples, where band-intensity changes serve as a read-out of PI3K-AKT pathway activity. The unconjugated format leaves labs free to pair it with HRP- or fluorophore-conjugated secondary antibodies, while the polyclonal backbone broadens coverage around the phospho-S256 mark for consistent detection across experimental conditions. Reactivity covers human and mouse.