Anti-FOXO1 (phospho S256) Antibody

A rabbit polyclonal IgG preparation raised against a synthetic phosphopeptide corresponding to the serine 256 residue of human FOXO1 (Forkhead box protein O1). The antibody selectively detects the phosphorylated form of the transcription factor in cell and tissue lysate workflows, making it a useful reagent for signaling studies focused on insulin, PI3K/AKT, and oxidative stress pathways where phosphorylation at Ser256 modulates FOXO1 nuclear export and transcriptional activity on downstream targets including G6PC, PEPCK, and cyclin D1. The unconjugated format provides flexibility for researchers building custom detection schemes, including secondary HRP or fluorophore conjugation for chemiluminescent Western Blotting applications. Validated reactivity covers Human and Mouse samples, supporting cross-species comparative work in metabolic disease, immunology, and cancer biology models. The reagent is supplied as 200 µl of liquid stock in a vial; for long-term stability, aliquot to avoid repeated freeze-thaw cycles and store at -20°C. This Antibodies.com antibody pairs effectively with total FOXO1 antibodies for phospho/total ratio normalization in quantitative Western Blot analysis.