Anti-FoxO1 Antibody

Raised in rabbit against the forkhead box transcription factor FoxO1, this polyclonal primary antibody from Antibodies.com is supplied as an unconjugated 100 µl vial for western blot, immunohistochemistry, immunofluorescence, and ELISA workflows. The reagent cross-reacts with Human, Mouse, and Rat samples, supporting comparative work across standard cellular and animal models. FoxO1 is a ~70 kDa protein that shuttles between the nucleus and cytoplasm; Akt-mediated phosphorylation at Thr24, Ser256, and Ser319 drives its nuclear export and inactivation, linking insulin and growth-factor signaling to gluconeogenic gene expression, cell cycle arrest, and apoptosis. On western blot, the antibody detects the endogenous FoxO1 band in lysates from human, mouse, and rat cells, while in IHC and IF it labels FoxO1 in fixed tissue sections and cultured cells. The reagent should be stored at -20 °C, with working aliquots prepared to limit repeated freeze-thaw cycles, and retains activity across a 12 month shelf life when handled this way. Each lot is QC-tested to confirm specificity for FoxO1 in lysates from stimulated and unstimulated human cell lines. Investigators working on hepatic glucose metabolism, lymphocyte homeostasis, adipocyte differentiation, or tumor-suppressor pathways can use this antibody to track endogenous FoxO1 levels and its post-translational regulation.