Anti-FoxO1 Antibody

This polyclonal antibody from Antibodies.com targets FoxO1 (Forkhead box protein O1), a transcription factor that acts as a key downstream effector of the PI3K/Akt signaling pathway. FoxO1 regulates genes controlling gluconeogenesis, lipogenesis, apoptosis, oxidative stress responses, and cell cycle arrest, making it a focus of research in metabolism, longevity, diabetes, and oncology.

The unconjugated rabbit antibody is supplied as 100 µl of polyclonal antiserum validated for western blot, immunohistochemistry, immunofluorescence, and ELISA workflows. Reactivity has been confirmed against human, mouse, and rat samples, supporting cross-species investigation of FoxO1 expression and phosphorylation status in cell lines and primary tissues.

Because FoxO1 translocates between the cytoplasm and nucleus in response to insulin, growth factors, and cellular stress, researchers use this reagent to track nuclear-cytoplasmic shuttling, monitor Akt-mediated phosphorylation at consensus sites such as Thr24, Ser256, and Ser319, and probe downstream transcriptional programs. Aliquoting on first use and limiting freeze-thaw cycles helps preserve antibody activity over long-term storage.