Anti-FOXD3 Antibody

Supplied as 100 µl of unconjugated reagent from Antibodies.com, this rabbit polyclonal antibody specifically recognizes FOXD3, a forkhead/winged-helix transcription factor central to early embryogenesis, neural crest specification, and the maintenance of pluripotency in stem cell populations. The unconjugated format provides flexibility for users who wish to apply their own detection chemistries, fluorophore conjugations, or HRP-based secondary systems.

Validated for Western blot and immunohistochemistry, the antibody shows reactivity with both human and mouse samples. Researchers apply FOXD3 detection in developmental biology workflows, neural crest lineage tracing, melanoma biology studies, and stem cell characterization projects, where the protein serves as a useful marker for tumor cell plasticity and melanocyte differentiation experiments.

The polyclonal format offers broader epitope coverage than monoclonal alternatives, increasing the probability of consistent detection across different protein isoforms, post-translational modifications, or fixation conditions. Typical applications include probing whole-cell and nuclear lysates by western blot, and localizing FOXD3 expression in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry. Brief centrifugation before aliquoting helps recover material from the vial, and the reagent should be stored refrigerated and protected from light to preserve activity. For research use only.