Anti-FOXD3 Antibody

Raised in rabbit against the Forkhead Box D3 transcription factor, this polyclonal IgG reagent from Antibodies.com is supplied as an unconjugated 50 µl vial optimized for Western Blot detection of FOXD3 in Human and Mouse samples. It targets the full-length FOXD3 protein, a winged-helix/forkhead domain transcription factor best known for its role in maintaining pluripotency in early embryonic stem cells, regulating neural crest cell specification, and controlling melanocyte lineage commitment. Generated as a polyclonal reagent, the antibody binds multiple epitopes across FOXD3, which is useful when probing conformational or partially denatured targets or when cross-reactivity between closely related forkhead family members is a concern. The reagent is used in stem cell reprogramming studies, melanoma biology, and neural crest-derived developmental research to characterize FOXD3 expression by immunoblotting of whole-cell or nuclear lysates. The unconjugated format allows downstream flexibility: secondary antibodies conjugated to HRP, fluorophores, or biotin can be paired with the primary antibody to match chemiluminescence or fluorescence detection workflows. As an IgG isotype selected for affinity purification, the reagent is suited for reproducible band detection under standard reducing SDS-PAGE conditions.