Anti-FLT3 (Ab-591) Antibody

Raised in rabbit against a synthetic peptide spanning the region around amino acids 589–593 (Y-F-Y-V-D) of human FLT3, this polyclonal antibody from Antibodies.com is supplied as an unconjugated, affinity-purified reagent at a concentration of 1.0 mg/mL in a 50 µl volume. It is validated for Western blotting (WB) and immunocytochemistry/immunofluorescence (ICC/IF) on human samples, where it recognises the endogenous total FLT3 protein without requiring a phosphorylated epitope. The host species is Rabbit, the clonality is Polyclonal, and the conjugate is Unconjugated, making it suitable for users who want to pair the reagent with their own secondary label or detection chemistry. The formulation is phosphate-buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4, containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol as a cryoprotectant. According to the supplier's storage guidance, the vial is shipped at 4 °C and should be aliquoted and stored at –20 °C upon arrival, with repeated freeze/thaw cycles avoided to preserve activity. Standard recommended dilutions are 1:500–1:1000 for Western blot and 1:100–1:200 for immunofluorescence, consistent with the validation data shown for 293 and MCF cell lysates. For research use only; not intended for diagnostic or therapeutic applications.