Anti-FLI1 Antibody

Manufactured by Antibodies.com, this affinity-purified polyclonal reagent is raised in rabbit against the Friend leukemia integration 1 transcription factor (FLI1) and is supplied as a 50 µL aliquot at a working concentration of 1.0 mg/mL in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. The unconjugated antibody was generated using a recombinant human FLI1 immunogen and is validated for Western blotting, immunohistochemistry, and immunocytochemistry/immunofluorescence workflows, detecting the endogenous level of total FLI1 protein. It cross-reacts with human, mouse, and rat samples, making it a versatile option for cross-species studies in hematopoiesis, Ewing sarcoma, and vascular biology research. For handling, the vial is shipped at 4 °C and should be aliquoted upon arrival for long-term storage at −20 °C, with repeated freeze/thaw cycles avoided to preserve binding activity. Researchers can pair this primary antibody with their own HRP- or fluorophore-labeled secondary reagents for chromogenic or fluorescence detection, and the polyclonal format provides robust recognition of multiple epitopes on the target transcription factor.