Anti-Flg Antibody

Raised in rabbit and affinity-purified by epitope-specific immunogen chromatography, this unconjugated polyclonal reagent from Antibodies.com is designed for sensitive detection of endogenous Flg protein. Provided as a 50 µL liquid formulation at 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, the antibody was generated against a synthesized peptide derived from human Flg around the non-phosphorylation site of Y654. It is validated for ELISA, Western blot (WB) and immunocytochemistry/immunofluorescence (ICC/IF), and cross-reacts with Human, Mouse and Rat samples, making it a versatile choice for comparative studies across standard model systems. Performance has been demonstrated by Western blot analysis of 293 cell lysates, supporting reliable detection of the endogenous target. For handling, the vial is shipped at 4 °C and should be aliquoted upon arrival and stored at −20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Intended for research use only, this Rabbit polyclonal Anti-Flg Antibody is well suited to laboratories investigating filaggrin-related pathways in skin biology, barrier function and related signalling research.