Anti-FKHR (Ab-319) Antibody

From the Antibodies.com catalog of validated research reagents, this rabbit polyclonal antibody targets the FKHR (FOXO1) protein at the non-phosphorylated region surrounding serine 319 and is supplied as a 50 µl liquid preparation at 1 mg/ml. The reagent is formulated in phosphate-buffered saline (pH 7.4, without Mg²⁺ and Ca²⁺) containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol, and was affinity-purified from rabbit antiserum by epitope-specific affinity chromatography. It is unconjugated, of the IgG isotype, and is reactive with human and mouse samples, making it a versatile tool for studies of forkhead box transcription factor signaling. The antibody is validated for Western blot (recommended dilution 1:500–1:1000) and immunohistochemistry (1:50–1:100), where it specifically detects endogenous levels of total FKHR protein. For routine handling, the vial is shipped at 4 °C and should be stored at –20 °C for extended periods; aliquoting is advised to avoid repeated freeze–thaw cycles. Researchers investigating the FOXO1 / FOXO1A pathway, forkhead-driven transcription, insulin and growth-factor signaling, or rhabdomyosarcoma biology can rely on this Antibodies.com polyclonal to deliver consistent, published-quality performance across standard immunodetection workflows.