Anti-FKHR (Ab-319) Antibody

Produced by Antibodies.com, this rabbit polyclonal antibody specifically recognizes the FKHR transcription factor (also known as FOXO1) when phosphorylated at serine 319, a regulatory site controlled by AKT/PKB and other upstream kinases. The reagent is supplied at 100 µl in an unconjugated format, ready to be paired with a secondary antibody of the user's choice for chromogenic or fluorescent detection.

Validated for Western blot (WB) and immunohistochemistry (IHC), the antibody reacts with both Human and Mouse samples, enabling comparative studies across cultured cell lines, primary tissues, and animal model specimens. Common applications include monitoring insulin and IGF-1 signaling output, tracking FOXO1 nuclear-cytoplasmic shuttling, and assessing apoptosis regulation in metabolic disease, oncology, and aging research.

Key technical details:

• Host: Rabbit
• Clonality: Polyclonal
• Conjugate: Unconjugated
• Target: FKHR/FOXO1 phospho-Ser319
• Volume: 100 µl
• Research use only

For storage, aliquot upon arrival to limit freeze-thaw cycles and store refrigerated at 2-8 degrees C. Each lot is quality-controlled to support reproducible band detection at the expected molecular weight on Western blot and specific cytoplasmic or nuclear staining patterns in IHC.