Anti-FKBP8 Antibody

This unconjugated rabbit polyclonal IgG antibody targets FKBP8, a mitochondrial-membrane-localized immunophilin of the FKBP family that regulates apoptosis through Bcl-2 binding and participates in mitophagy by associating with LC3. Generated by Antibodies.com in a 100 µl volume, the reagent is validated across three orthogonal readouts that interrogate distinct molecular contexts: Western blot for expression-level analysis in whole-cell lysates, immunofluorescence for subcellular localization studies, and immunoprecipitation for interaction-partner mapping such as Bcl-2 or LC3 complexes. The antibody recognizes both the human and murine FKBP8 orthologs, supporting parallel work in cell-line panels and primary rodent tissue. Polyclonal reactivity across multiple epitopes enables reliable detection even when the antigen presents conformational variation, an advantage when probing native mitochondrial preparations, autophagy-associated fractions, or post-translational modification states. The unconjugated format permits direct pairing with user-chosen reporters, including HRP or biotin conjugates for chemiluminescent Western blot detection, fluorophore-labeled secondaries for confocal fluorescence microscopy, and bead-based chemistries for pull-down assays. For best results, aliquot upon receipt to limit repeated freeze-thaw cycles and store refrigerated to preserve polyclonal titer; small working aliquots held cold remain stable through extended experimental campaigns. Researchers examining mTOR pathway biology, mitochondrial chaperone function, or programmed cell death will find this reagent straightforward to integrate into standard immunodetection pipelines, particularly when paired with a species-matched secondary antibody and validated positive- and negative-control lysates.