Anti-Filamin B Antibody

Raised in rabbit against the cytoskeletal linker Filamin B, this unconjugated polyclonal reagent from Antibodies.com is supplied at 100 µl and validated for Western blot (WB) and immunofluorescence (IF) on human samples. The antibody binds an actin-cross-linking protein that organizes the submembranous actin network and scaffolds signaling partners such as integrins and small GTPases, supporting studies of cell shape, migration, and mechanotransduction. Filamin B is also implicated in skeletal development, and pathogenic variants in the encoding gene cause disorders including Larsen syndrome, spondylocarpotarsal synostosis, and boomerang dysplasia, so the reagent is useful in disease-model research. As an unconjugated polyclonal, the antibody gives researchers flexibility in secondary pairing for chromogenic WB and fluorescent IF detection, while the polyclonal format increases the chance of recognizing multiple epitopes on the FLNB protein and its isoforms. Recommended starting points include RIPA-based lysate preparation for WB and 4% paraformaldehyde fixation for IF on adherent cell lines such as fibroblasts or osteoblasts.