Anti-Fibulin-1 Antibody

This affinity-purified polyclonal reagent from Antibodies.com is raised in rabbit against the extracellular matrix glycoprotein Fibulin-1 (FBLN1) and is supplied as a 50 µl liquid aliquot at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol as a cryoprotectant. The unconjugated IgG preparation is validated for Western blot detection of endogenous Fibulin-1 across human, mouse and rat samples, making it suitable for cross-species expression studies in cell and tissue lysates. As a polyclonal antibody, it offers broad epitope coverage of the 77 kDa target, and the standard PBS/glycerol formulation is ready to dilute directly into blocking buffer at typical WB working ranges (commonly 1:500–1:2,000 depending on antigen abundance). Researchers should handle the vial on ice, avoid repeated freeze–thaw cycles, and store the glycerol-containing stock at –20 °C for long-term stability, with short-term use at 4 °C. The unconjugated format allows end-users to pair the primary reagent with their preferred HRP, fluorophore or biotin secondary detection system, providing flexibility for chemiluminescent or fluorescent Western blot workflows. With reactivity to three commonly used model species and a high-concentration formulation, this Fibulin-1 antibody is well suited for comparative studies of basement-membrane and elastic-fibre biology, fibulin-1C/D isoform analysis, and investigations into fibulin-1 involvement in cardiovascular, ocular and tumour-stroma research.