Anti-FGL1 Antibody

This rabbit polyclonal primary antibody from Antibodies.com targets Fibrinogen-like protein 1 (FGL1), a liver-enriched secretory glycoprotein also known as Hepassocin. FGL1 has attracted substantial research interest as the major functional ligand of the LAG-3 immune checkpoint receptor, linking it to T-cell exhaustion pathways and tumor immune evasion studies in hepatocellular carcinoma, melanoma, and lung cancer models. The unconjugated IgG reagent is supplied as 200 µl of polyclonal antiserum raised in rabbit, validated for Western Blot detection of endogenous FGL1 in human, mouse, and rat samples. Researchers apply the antibody to whole-cell lysates and tissue homogenates to investigate FGL1 expression regulation, its secretion dynamics under metabolic stress, and competitive binding interactions with recombinant LAG-3 fusion proteins. Storage at -20°C in working aliquots preserves activity across repeated freeze-thaw cycles, and the polyclonal format provides recognition of multiple epitopes within the fibrinogen-related domain, an advantage for resolving full-length and truncated isoforms that emerge from alternative splicing. Intended for research use only, the antibody pairs readily with standard HRP- or fluorophore-conjugated secondary reagents for chemiluminescent or fluorescent Western Blot readouts.