Anti-FGFR1 (phospho-Y654) Antibody

Phosphorylation of fibroblast growth factor receptor 1 at tyrosine 654 marks the activated state of this receptor tyrosine kinase, and this rabbit polyclonal antibody from Antibodies.com enables targeted recognition of that modified form in cell and tissue lysates. Raised in rabbit and purified against a phospho-Y654 containing immunogen, the reagent is supplied as 100 µl of a 1 mg/ml stock in PBS containing 0.1% sodium azide and 50% glycerol.

The unconjugated antibody recognizes FGFR1 when phosphorylated at Y654 in human, mouse, and rat samples and is validated for Western blot. Investigators studying FGFR1-driven signaling use it to monitor receptor activation kinetics after FGF ligand stimulation, to compare basal and induced phosphorylation across cancer cell lines, and to follow turnover of the activated receptor. Storage at -20 °C in small aliquots is recommended to limit freeze-thaw cycles, and the reagent is intended for research use only.