Anti-FGF2 Antibody

FGF2 (basic fibroblast growth factor, bFGF) is a heparin-binding mitogen central to angiogenesis, wound healing, and mesodermal cell proliferation. This unconjugated rabbit polyclonal antibody from Antibodies.com is supplied as 50 µl of liquid stock and has been validated for Western blot and immunohistochemistry on human samples.

Target background

FGF2 (also called HBGF-2 or FGF-β) is a non-glycosylated member of the FGF growth factor family that signals through FGFR1–FGFR4 receptors to drive endothelial cell migration, fibroblast expansion, and stem cell maintenance. Aberrant FGF2 expression is linked to tumor vascularization and chronic inflammatory tissue remodeling, which is why FGF2 is widely tracked in cancer biology and regenerative medicine research.

Validated applications

• Western blot detection of endogenous FGF2 from human cell lysates, including the canonical ~18 kDa low-molecular-weight isoform and the higher-molecular-weight nuclear-localized forms.
• Immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections, where the polyclonal reagent helps amplify signal from the relatively small FGF2 protein.

Format and handling

Host: Rabbit. Clonality: Polyclonal. Conjugate: Unconjugated. Reactivity: Human. Each vial contains 50 µl of antiserum; aliquot upon receipt and freeze at -20°C in single-use working volumes to limit freeze-thaw cycles. Working dilutions should be titrated against an endogenously FGF2-expressing control such as HeLa or HUVEC lysate to set the optimal signal-to-background balance for the assay of choice.