Anti-FEN1 (7H8) Antibody

This mouse monoclonal antibody from Antibodies.com targets human FEN1 (Flap Endonuclease 1), a structure-specific metallonuclease critical for Okazaki fragment maturation during lagging-strand DNA replication and for long-patch base excision repair. Clone 7H8 is supplied as 100 µl of purified immunoglobulin in a stabilizing buffer containing 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% sodium azide, and 50% glycerol.

Validated for Western blot and immunocytochemistry, the reagent cross-reacts with mouse and rat FEN1 in addition to the human protein, supporting comparative studies across common model systems. Researchers use clone 7H8 to investigate replication fork processing, the DNA damage response, and the role of FEN1 dysregulation in tumor proliferation and chemoresistance.

Store the vial at -20°C in the supplied buffer and limit freeze-thaw cycles to preserve activity. The unconjugated format allows flexible pairing with secondary reagents for fluorescence, chromogenic, or chemiluminescent detection workflows.