Anti-FBXW5 Antibody

Antibodies.com offers a Rabbit polyclonal antibody to FBXW5, raised in Rabbit and supplied as an unconjugated IgG preparation validated for Western blot on human samples. The target is FBXW5, with a stated molecular weight of 72 kDa [C0][S1][S2].

The immunogen is a recombinant fusion protein corresponding to amino acids 184–566 of human FBXW5 (NP_061871.1) [S1][S2]. The antibody is affinity purified and supplied as a liquid in Phosphate Buffered Saline, pH 7.3, containing 50% glycerol and 0.01% Thiomersal [S1][S2]. Recommended usage is WB at 1:200–1:1,000 [S1][S2]. Storage instructions call for shipping at 4°C with aliquoting and storage at -20°C, avoiding freeze/thaw cycles [S1][S2].

Validation is supported by a Western blot analysis of A-549 cell extracts performed at 1:1,000 dilution, using a Goat Anti-Rabbit IgG H&L HRP secondary at 1:10,000, with 25 µg lysate per lane, 3% non-fat dry milk in TBST blocking, ECL Basic Kit detection, and a 5 second exposure [S2].