Anti-Fatty Acid Synthase Antibody

Fatty Acid Synthase (FASN) is a homodimeric multifunctional enzyme that catalyzes the de novo biosynthesis of saturated long-chain fatty acids, primarily palmitate, from acetyl-CoA and malonyl-CoA. The enzyme, with subunits around 270 kDa, has emerged as a focal target in studies of tumor lipogenesis, metabolic disease, and adipocyte differentiation. Antibodies.com supplies this unconjugated rabbit polyclonal reagent at 100 µl per vial, validated for Western blot, immunohistochemistry, and immunofluorescence applications on human samples.

Researchers apply the antibody to detect endogenous FASN expression in cell lysates, formalin-fixed tissue sections, and cultured cell lines. Common experimental contexts include tracking lipogenic reprogramming in cancer cell models, profiling metabolic shifts in obesity and diabetes research, and confirming FASN knockdown or overexpression in genetic studies. Because the reagent is unconjugated, laboratories can pair it with HRP-conjugated, biotinylated, or fluorophore-labeled secondary antibodies, supporting both chromogenic and fluorescence detection workflows.

Polyclonal antibodies recognize multiple epitopes on the target protein, which generally produces stronger signals in Western blot and broader tolerance for partial protein denaturation in IHC. Recommended handling includes brief refrigerated storage for active use, with longer-term storage at -20 °C in working aliquots to limit freeze-thaw damage to the immunoglobulin. The 100 µl volume accommodates multiple pilot experiments and ongoing study use.