Anti-FARSLB Antibody

Raised in rabbit against the beta subunit of phenylalanyl-tRNA synthetase (FARSLB, also called FARSB), this unconjugated polyclonal reagent recognizes a cytosolic aminoacyl-tRNA synthetase that charges tRNA^Phe during cytoplasmic translation. Antibodies.com supplies the antibody at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, supplied as a 100 µl aliquot suitable for routine immunoblotting workflows on PVDF or nitrocellulose membranes.

Validated for western blot detection of endogenous FARSLB, the antibody cross-reacts with human, mouse, and rat samples, enabling comparative studies of phenylalanyl-tRNA charging capacity across mammalian cell lines and primary cultures. Polyclonal recognition of multiple epitopes makes it useful for tracking full-length expression of this housekeeping synthetase and for studies of translation-related disease models where FARSB levels or complex assembly are of interest.

Working dilution should be titrated empirically; storage at 4 °C is appropriate for short-term use, while longer term storage as frozen aliquots helps preserve activity across multiple freeze-thaw cycles. The unconjugated format allows pairing with HRP-, AP-, or fluorophore-conjugated secondaries chosen by the user, giving flexibility for chemiluminescent, colorimetric, or fluorescent readout platforms in standard research use only settings.