Anti-FANCG Antibody

This Rabbit polyclonal IgG antibody from Antibodies.com is raised against FANCG and is supplied as an unconjugated liquid preparation validated for Western blot (WB). The antibody is reactive with Human and Mouse samples and has been affinity purified. The target protein FANCG has a reported molecular weight of 69 kDa.

The immunogen is a recombinant fusion protein corresponding to amino acids 343-622 of human FANCG (NP_004620.1). The formulation is Phosphate Buffered Saline at pH 7.3, supplemented with 50% glycerol and 0.02% sodium azide. For Western blot, the recommended dilution range is 1:500-1:2,000. The antibody is shipped at 4°C; upon delivery it should be aliquoted and stored at -20°C, with repeated freeze/thaw cycles avoided.

Validation data are provided for Western blot analysis of extracts from various cell lines, performed at 1:1,000 dilution, with a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at 1:10,000. Lysates were loaded at 25 µg per lane, blocked with 3% non-fat dry milk in TBST, and detected using an ECL Basic Kit with a 50-second exposure. The product is intended for research use only and is not for diagnostic or therapeutic use.