Anti-FAK (Phospho-Tyr925) Antibody

A rabbit polyclonal antibody from Antibodies.com that recognizes FAK only when phosphorylated at tyrosine 925. The reagent is unconjugated and has been validated for Western blotting (WB) and immunocytochemistry/immunofluorescence (ICC/IF), reacting with samples from Human, Mouse, and Rat.

It was raised against a synthetic phosphopeptide corresponding to the sequence around the tyrosine 925 phosphorylation site of human FAK (K-V-Y(p)-E-N), and purified by affinity chromatography against the epitope-specific phosphopeptide, with non-phospho-specific antibodies removed using the non-phosphopeptide. The antibody is supplied at 1.0 mg/mL in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol, and is shipped at 4°C for long-term storage at -20°C with freeze/thaw cycles to be avoided.