Anti-FAK Antibody

The antibody targets Focal Adhesion Kinase (FAK, also designated PTK2), a 125 kDa non-receptor protein tyrosine kinase that localizes to focal adhesions and integrates signals from integrins and growth factor receptors. Raised in rabbit and supplied as a polyclonal reagent, this unconjugated primary antibody from Antibodies.com is offered in a 100 µl volume and is validated for Western blot, immunohistochemistry, and ELISA workflows.

In Western blot applications, it detects the native FAK protein near its expected molecular weight of approximately 125 kDa, supporting studies of integrin signaling, cytoskeletal remodeling, and mechanotransduction. IHC use permits visualization of FAK distribution in tissue sections from human, mouse, and rat samples, while ELISA compatibility enables quantitative measurement of FAK levels across experimental conditions.

The unconjugated format gives researchers flexibility to conjugate the antibody to their preferred reporter or to use it directly with secondary detection reagents. For long-term use, the antibody should be stored frozen in single-use aliquots to limit freeze-thaw cycles and preserve binding activity. Researchers investigating focal adhesion dynamics, tumor cell migration, or downstream phosphorylation events such as autophosphorylation at tyrosine residue 397 will find this reagent useful across in vitro cell biology and tissue-based studies.