Anti-F9 Antibody

This rabbit polyclonal antibody from Antibodies.com targets coagulation factor IX (F9), a vitamin K-dependent serine protease that drives the intrinsic pathway of blood coagulation. Loss-of-function mutations in F9 cause hemophilia B (Christmas disease), an X-linked bleeding disorder, so reliable immunological detection of the protein underpins research into clotting factor biology, gene-therapy constructs, and hematology workflows.

The reagent is supplied as 100 µl at 1 mg/ml in phosphate buffered saline containing 15 mM sodium azide at approximately pH 7.2. The unconjugated format gives researchers flexibility to pair it with their own secondary detection reagents and enzyme or fluorophore labeling strategies. Host species is rabbit, clonality is polyclonal, and the antibody has been validated for Western blot (WB) application with reactivity confirmed in human and mouse samples, covering both clinical specimens and the standard rodent models used to study FIX expression.

Polyclonal recognition of multiple epitopes on F9 can be helpful in Western blot workflows where denatured, reduced protein is separated by SDS-PAGE, since several binding regions remain accessible. Sodium azide in the formulation acts as a preservative to protect reagent integrity during routine benchtop handling. The product is packaged as a single-use vial of 100 µl, providing ample material for small pilot experiments and validation studies that compare F9 levels across treated and untreated cohorts.