Anti-F5 Antibody

This rabbit polyclonal reagent from Antibodies.com targets Coagulation Factor V (F5), a large liver-derived glycoprotein that serves as an essential cofactor within the prothrombinase complex during the blood coagulation cascade. The antibody is supplied as 100 µl of a 1 mg/ml stock in phosphate buffered saline (PBS) containing 15 mM sodium azide, at approximately pH 7.2, in a single vial.

Validated for Western blot on human and rat samples, the unconjugated immunoglobulin supports investigation of haemostasis, thrombosis, and inherited Factor V deficiency (Owren parahemophilia), as well as studies of hepatic protein synthesis and bleeding diatheses. The polyclonal format amplifies signal through recognition of multiple epitopes on the target, which is helpful when endogenous F5 expression is modest in cell or tissue lysates.

For handling, refrigerate the vial on arrival to preserve activity; the sodium azide serves as a microbial preservative in the formulation. Aliquot upon first use to limit freeze-thaw cycles of the working stock. The unconjugated format gives researchers flexibility to pair the reagent with a secondary antibody of their choice, including HRP- or fluorophore-labelled secondaries for chemiluminescent or fluorescent Western blot detection.