Anti-F12 Antibody

Manufactured by Antibodies.com, this unconjugated rabbit polyclonal antibody was raised against coagulation Factor XII (F12), a serine protease that initiates the intrinsic pathway of contact activation. Purified from rabbit serum and supplied in a 100 µl vial, the reagent is validated for western blot and immunohistochemistry workflows and reacts with human, mouse, and rat samples, supporting cross-species comparison in basic research.

Factor XII sits at the head of the intrinsic coagulation cascade, where surface-contact autoactivation drives downstream kallikrein and kinin signalling, bradykinin release, and fibrin formation. Researchers working on thrombosis models, contact-system pharmacology, vascular inflammation, and hereditary angioedema mechanisms use F12-targeting antibodies to probe protein expression across tissue lysates and fixed sections.

Provided unconjugated, the antibody accepts any compatible secondary reporter of choice, enabling chromogenic, fluorescent, or chemiluminescent detection in diverse experimental platforms. Aliquoting upon receipt and storing frozen protects activity through repeated freeze-thaw cycles, extending the usable shelf life for long-running studies.