Anti-EZR (phospho Y353) Antibody

This rabbit polyclonal IgG antibody from Antibodies.com is raised against Ezrin (EZR) phosphorylated at tyrosine 353, a regulatory site located in the helical linker region between the N-terminal FERM domain and the C-terminal actin-binding domain. Phosphorylation at Y353 by Src-family kinases is implicated in cytoskeletal remodeling, cell migration, and metastatic behavior, making phospho-specific detection relevant to studies of tumor invasion and motility. The unconjugated antibody is supplied as a 50 µl vial and is validated for Western blot and immunohistochemistry on human and mouse specimens, providing a tool to monitor EZR activation state in lysed or fixed tissue preparations. For storage, aliquot and store at -20 °C; limit freeze-thaw cycles to preserve phospho-epitope recognition.