Anti-ETFA Antibody

A primary antibody from Antibodies.com produced as a rabbit polyclonal IgG directed against ETFA. The reagent is unconjugated and validated for reactivity with human, mouse and rat samples. The catalogue entry lists Western blot, immunohistochemistry and immunofluorescence among its applications, while the manufacturer datasheet lists Western blot, immunohistochemistry and immunoprecipitation.

The immunogen was a recombinant fusion protein corresponding to amino acids 1-333 of human ETFA (NP_000117.1). The antibody is affinity-purified and supplied as a liquid in Phosphate Buffered Saline (pH 7.3) containing 50% glycerol and 0.02% sodium azide. Reported molecular weight is 35 kDa and concentration is lot specific. Storage guidance is shipment at 4°C, with aliquoting and storage at -20°C on receipt and avoidance of freeze/thaw cycles.

Recommended working dilutions (manufacturer datasheet) are WB 1:500-1:2,000, IHC 1:50-1:200 and IP 1:500-1:1,000. The manufacturer reports eight scientific validation images supporting these uses.