Anti-ET-1 Antibody

Endothelin-1 (ET-1) is a 21-amino acid vasoactive peptide produced primarily by vascular endothelial cells, where it acts as one of the most potent endogenous vasoconstrictors known. This unconjugated polyclonal reagent from Antibodies.com is raised in rabbit hosts and binds the human ET-1 sequence, supporting detection of the mature peptide and its precursor intermediates. Working applications include Western blot analysis of cell and tissue lysates alongside ELISA-based quantification of peptide levels. The unconjugated format leaves the choice of secondary label open, allowing flexible pairing with HRP, fluorophore, or biotin conjugates as the experimental design requires. Each vial ships as a 100 µl liquid aliquot ready for dilution into standard immunoblotting or immunoassay buffers, and the polyclonal profile helps capture transient or low-abundance epitopes on the endothelin precursor. Studies in vascular biology, pulmonary hypertension, and cardiovascular disease frequently rely on this reagent to localize ET-1 production in cultured cells, frozen sections, and tissue homogenates.