Anti-Estrogen Receptor Antibody [H17-A]

This Rabbit monoclonal antibody, clone H17-A, from Antibodies.com provides specific detection of the human Estrogen Receptor alpha (ERα, NR3A1) nuclear hormone receptor. Conjugated to FITC for direct fluorescent readout, the reagent is supplied as a liquid formulation sized for 100 tests and is validated for flow cytometry (FC) on human cells. The IgG isotype reagent targets a conserved epitope of the N-terminal transactivation domain of ERα, making it useful for identifying ER-positive populations in breast cancer cell lines such as MCF-7, characterizing hormone-responsive models, and sorting estrogen receptor-expressing populations by immunofluorescence. The H17-A clone has a long history of use in nuclear receptor studies, originating from one of the reference antibody lineages used to characterize ERα expression, localization, and trafficking in fixed and permeabilized cells. As an FITC conjugate, the reagent should be protected from light during handling and storage, and is intended for research use only.