Anti-Estrogen Receptor-α (Ab-537) Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against Estrogen Receptor-α phosphorylated at serine 537, allowing selective detection of the activated form of this nuclear hormone receptor. The reagent is supplied as 100 µl of unconjugated antiserum in a single vial and is validated for Western blot and immunohistochemistry on human and mouse specimens.

Estrogen Receptor-α mediates estrogen-driven signaling in breast, endometrial, and other hormone-responsive tissues. Phosphorylation at Ser537 has been linked to ligand-independent receptor activation that contributes to endocrine resistance in breast cancer models, so a phosphorylation-state-specific reagent distinguishes the kinase-active pool from total ERα in tumor biopsies, cell line lysates, and paraffin-embedded tissue sections.

The polyclonal rabbit host gives broader epitope coverage than a monoclonal reagent, which is useful when probing low-abundance phospho-ERα signals. Aliquot the antiserum on first use and store at -20 °C, avoiding repeated freeze-thaw cycles to preserve antibody titer. For research use only.