Anti-Escherichia coli (E. coli) Antibody

Raised in goats and affinity-purified to an immunoglobulin fraction, this unconjugated polyclonal reagent enables detection of Escherichia coli antigens across multiple immunoassay workflows. Supplied by Antibodies.com as a 500 µl liquid aliquot, the antibody recognizes whole-cell and lysate preparations of the target bacterium, supporting routine identification of this Gram-negative organism in microbiology and contamination-screening contexts.

The reagent is validated for ELISA, immunofluorescence assay (IFA), and Western blot, allowing laboratories to use the same primary antibody across plate-based antigen capture, microscopy-based visualization, and immunoblot confirmation of expressed proteins. Its unconjugated format gives end users flexibility to label the reagent themselves with HRP, biotin, fluorophores, or gold conjugates depending on the assay design. As a polyclonal reagent, the antibody recognizes multiple epitopes on E. coli structural components, which improves signal strength on heterogeneous or partially degraded bacterial samples.

For long-term integrity, the product should be handled refrigerated in working aliquots to limit freeze-thaw cycles and preserve functional binding activity. The 500 µl fill volume supports dozens of typical staining or blotting reactions, making the reagent a practical choice for research laboratories, food safety testing facilities, veterinary diagnostic labs, and academic core facilities engaged in pathogen characterization.