Anti-ERK5 Antibody

ERK5 (also called MAPK7 or BMK1) is the largest member of the MAP kinase family, distinguished by a long C-terminal extension that drives transcription factor activation downstream of MEK5 signaling. This rabbit polyclonal antibody from Antibodies.com is raised against ERK5 and validated across three immunodetection workflows: western blot analysis of cell and tissue lysates, immunohistochemistry on fixed sections, and immunofluorescence imaging. The reagent is supplied as a 100 µl aliquot at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, unconjugated so investigators can pair it with secondary antibodies matching their preferred detection chemistry. Cross-reactivity is confirmed against human, mouse, and rat ERK5, supporting comparative studies in primary cells and rodent model systems. In western blotting, the antibody highlights the high molecular weight full-length ERK5 band and detects the truncated C-terminal cleavage product that accumulates in the nucleus following stress or growth-factor stimulation. Storage at -20°C preserves activity, and the glycerol-containing buffer maintains antibody integrity during routine cold-storage handling and brief freeze-thaw events.