Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) Antibody

A rabbit polyclonal antibody from Antibodies.com recognises ERK1 phosphorylated at Thr202 and Tyr204, alongside ERK2 phosphorylated at Thr185 and Tyr187. The reagent is an unconjugated IgG raised in rabbit and purified by affinity chromatography. According to the Antibodies.com listing, validated applications are Western blot, immunohistochemistry and immunoprecipitation, with reactivity reported against human, mouse and rat samples. The manufacturer specification additionally lists Western blot and immunocytochemistry/immunofluorescence, with reactivity stated for mouse and rat. The immunogen is a synthetic phosphorylated peptide around Thr185 and Tyr187 of human ERK2/MAPK1 (RefSeq NP_002736.3), sequence LTEYV. The reported molecular weights are 42 kDa and 44 kDa. The antibody is supplied as a liquid in Phosphate Buffered Saline at pH 7.3 containing 50% glycerol and 0.05% Proclin 300, at a lot-specific concentration. Suggested starting dilutions are 1:100–1:500 for Western blot and 1:50–1:200 for immunocytochemistry/immunofluorescence. The product is shipped at 4°C and should be aliquoted and stored at -20°C upon receipt, avoiding repeated freeze/thaw cycles.