Anti-ERK1/2 (Y204) Antibody

Raised in rabbit and affinity-purified, this unconjugated polyclonal reagent from Antibodies.com targets the phosphorylated tyrosine 204 (Y204) epitope of ERK1/2, a canonical activation site within the MAPK/ERK signalling cascade. The antibody recognizes both ERK1 (MAPK3) and ERK2 (MAPK1) isoforms and has been validated for Western blot, immunohistochemistry, and immunofluorescence workflows on human, mouse, and rat samples.

The immunogen corresponds to a region surrounding Y204, allowing selective detection of the activated, phosphorylated kinase rather than total ERK protein. Researchers commonly apply this reagent to monitor pathway activation downstream of growth factor, cytokine, or stress stimuli, and to study roles in proliferation, differentiation, and survival across cell and tissue models. Staining of fixed tissue sections by IHC and imaging by IF both benefit from the unconjugated format, which allows pairing with custom secondary labels as protocols require.

Each vial contains 100 µl of antibody at 1 mg/ml in Phosphate buffered saline (PBS) supplemented with 15 mM sodium azide, pH approximately 7.2. Aliquot before storage to limit freeze-thaw cycles, and refrigerate between uses; the sodium azide acts as a microbial preservative. This clone performs well on whole-cell lysates, paraffin-embedded sections, and cultured cells, supporting reproducible detection of phosphorylated ERK under basal and stimulated conditions.