Anti-ERK1/2 (L352) Antibody

Raised in rabbit against a synthetic peptide mapping to the L352 region of ERK1/2, this polyclonal antibody recognizes both the 44 kDa ERK1 and 42 kDa ERK2 isoforms central to the MAPK signaling cascade. Supplied by Antibodies.com as an unconjugated reagent at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, the 50 µl volume supports routine Western blot detection of total and phosphorylated ERK1/2 in cell lysates as well as immunohistochemical localization of the kinase in tissue sections.

Validated for reactivity with human, mouse, and rat samples, the antibody binds the conserved C-terminal epitope shared by ERK1 and ERK2, supporting pathway studies in cancer biology, neurodegeneration, and growth factor signaling research. The unconjugated format allows laboratories to pair the primary antibody with their preferred secondary reagent, HRP conjugate, or fluorophore for Western blot, immunofluorescence, or immunohistochemistry workflows, and the polyclonal response helps preserve recognition if minor denaturation occurs at the target site.